FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316977
Sequences flagged as poor quality0
Sequence length20
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT11290.3561772620726425No Hit
GGGTTTGGGTTTGGGTTTGG9590.3025456105648044No Hit
GGGGGGGGGGGGGGGGGGGG4430.13975777422336638No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT452.9875415E-514.0157961
TTCCTTT350.00100647114.0157961
TTCCTGG401.73076E-414.0157961
GTCCTGT558.984607E-714.0157961
CCTCTGT452.9875415E-514.0157961
CCTCTGG401.73076E-414.0157961
CTGCTGG401.73076E-414.0157961
CTTCTTT350.00100647114.0157961
CTTCTGT452.9875415E-514.0157961
CTTCTGG350.00100647114.0157961
CTCCTGG601.5640398E-714.0157951
TTCCTTG505.173686E-614.0157951
GTCCTTT601.5640398E-714.0157951
GTCCTGG601.5640398E-714.0157951
CTGCTTG300.00587860214.0157951
TCTCTGT505.173686E-614.0157951
GCTCTGT300.00587860214.0157951
CTTCTTG505.173686E-614.0157951
GCCCTGT300.00587860214.0157951
GCCCTGG300.00587860214.0157951
Produced by FastQC (version 0.11.5)