FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7245188
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT193650.26728084902696797No Hit
GGGTTTGGGTTTGGGTTTGG114790.15843619240798168No Hit
GGGGGGGGGGGGGGGGGGGG102390.1413213846210754No Hit
GTGGTTTTGTGTGTTTTTGT87680.1210182537706406No Hit
GTGGTTGTGTGGTGTTTTGT83590.11537312765383038No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG5650.014.0068991
CGCCTTT704.8785296E-914.0068991
CGCCTGT1400.014.0068991
CCTCGTG2900.014.0068991
GGCCGTG1350.014.0068991
CCGCTTG704.8785296E-914.0068991
GTCCGGT1350.014.0068991
CCTCGGG1250.014.0068991
CCGCTGG704.8785296E-914.0068991
TCGCGGT704.8785296E-914.0068991
TTCCTGG7950.014.0068991
CCTCTTG5400.014.0068991
CCCCGTT1350.014.0068991
CCTCTGG5800.014.0068991
GCGCTTG1450.014.0068991
CTTCGTG3850.014.0068991
CTGCTTG5000.014.0068991
TCCCGTG1350.014.0068991
CCCCTGT5000.014.0068991
CCCCTGG3350.014.0068991
Produced by FastQC (version 0.11.5)