FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918713
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG56250.14354202514958353No Hit
GTGTGTGTGTGTGTGTGTGT55490.14160261289867362No Hit
GGGTTTGGGTTTGGGTTTGG48450.12363753099550796No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT4550.014.0082751
CTCCTTG2600.014.0082741
CGCCTTT801.5097612E-1014.0082741
TGCCGTG453.0199051E-514.0082741
CTCCTGG4750.014.0082741
CGCCTGG1600.014.0082741
TTCCGGG1200.014.0082741
TGCCGGT559.115138E-714.0082741
TGCCGGG401.7466475E-414.0082741
CCGCTTT559.115138E-714.0082741
CCGCTTG801.5097612E-1014.0082741
CCTCGGT801.5097612E-1014.0082741
GTCCGGG1100.014.0082741
GGCCGGG652.7795977E-814.0082741
GCGCGTT453.0199051E-514.0082741
GCGCGTG1200.014.0082741
GCGCGGT300.00591539614.0082741
CTGCGTT652.7795977E-814.0082741
CCCCGTT401.7466475E-414.0082741
GGCCTGT2050.014.0082741
Produced by FastQC (version 0.11.5)