FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746088
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT83930.3056347793661383No Hit
GGGTTTGGGTTTGGGTTTGG40130.14613515663008614No Hit
GGGGGGGGGGGGGGGGGGGG33560.12221021321967832No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGG2900.014.0088931
CTCCTTG2550.014.0088921
TTCCGTG704.8639777E-914.0088921
TGCCGTG505.2351916E-614.0088921
CGCCTGG704.8639777E-914.0088921
TTCCGGG350.001013701814.0088921
GTCCGTT1400.014.0088921
CCTCGTT1200.014.0088921
CCGCTTT505.2351916E-614.0088921
CCTCGGT601.5885234E-714.0088921
GGCCGGT350.001013701814.0088921
CCGCTGT559.1077527E-714.0088921
CCGCTGG559.1077527E-714.0088921
TGCCTTT704.8639777E-914.0088921
TGCCTTG1200.014.0088921
TTCCTGG2450.014.0088921
TGCCTGG2050.014.0088921
GTCCTTT3450.014.0088921
CCTCTTT2250.014.0088921
GTCCTTG4800.014.0088921
Produced by FastQC (version 0.11.5)