FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398554
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT5710.14326791350732898No Hit
GGGGGGGGGGGGGGGGGGGG4640.11642086141401164No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG558.998413E-714.0162051
TTCCTGT704.780304E-914.0162051
TTCCTGG558.998413E-714.0162051
GTCCTTG852.5465852E-1114.0162051
GTCCTGT704.780304E-914.0162051
GTCCTGG852.5465852E-1114.0162051
GGCCTGG350.001007049514.0162051
CTGCTTT350.001007049514.0162051
CCCCTGG350.001007049514.0162051
TCTCTGG350.001007049514.0162051
GCTCTTG452.9905297E-514.0162051
GCTCTGG350.001007049514.0162051
CTTCTTT558.998413E-714.0162051
CTTCTGT452.9905297E-514.0162051
GCCCTGG558.998413E-714.0162051
CTCCTTG300.005880980314.0162041
TTCCTTG300.005880980314.0162041
TGCCTGT300.005880980314.0162041
GTCCTTT401.7320995E-414.0162041
CCTCTGT401.7320995E-414.0162041
Produced by FastQC (version 0.11.5)