FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701769
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG17990.10571352516117052No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG801.5097612E-1014.0064691
CGCCTGG1050.014.0064691
CCTCGGG401.7476431E-414.0064691
GCGCGTG401.7476431E-414.0064691
GGCCTTT801.5097612E-1014.0064691
TCTCGGT401.7476431E-414.0064691
TCGCTGG401.7476431E-414.0064691
CCCCTTG401.7476431E-414.0064691
CCCCTGT801.5097612E-1014.0064691
GCCCGTT401.7476431E-414.0064691
GCCCGGT401.7476431E-414.0064691
CTCCGTT401.7476431E-414.0064691
CTTCTGT2100.014.0064691
TCCCTTT401.7476431E-414.0064691
TCCCTTG401.7476431E-414.0064691
CTCCTTT1000.014.0064681
CGCCTTT505.241758E-614.0064681
CGCCTTG350.001014749814.0064681
TTCCGTT505.241758E-614.0064681
CTCCTGT1300.014.0064681
Produced by FastQC (version 0.11.5)