FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691233
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT42590.2518281041110243No Hit
GGGGGGGGGGGGGGGGGGGG27720.1639040865451419No Hit
GGGTTTGGGTTTGGGTTTGG24540.1451012367899633No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGT3900.014.0077231
GGCCTGT1950.014.0077231
GGCCTGG1950.014.0077231
CTCCTTT704.8639777E-914.0077211
CTCCTTG1600.014.0077211
CGCCTTT505.2368578E-614.0077211
TTCCGTT401.7463064E-414.0077211
TTCCGTG300.005915105314.0077211
CTCCTGT3000.014.0077211
TGCCGTG505.2368578E-614.0077211
CTCCTGG2400.014.0077211
CGCCTGT559.110354E-714.0077211
CGCCTGG505.2368578E-614.0077211
TGCCGGT453.0190284E-514.0077211
GTCCGTT801.4915713E-1014.0077211
CCTCGTT453.0190284E-514.0077211
GTCCGTG1200.014.0077211
CCTCGTG950.014.0077211
CCGCTTG401.7463064E-414.0077211
GTCCGGT1150.014.0077211
Produced by FastQC (version 0.11.5)