FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515430
Sequences flagged as poor quality0
Sequence length20
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT8410.16316473623964456No Hit
GGGGGGGGGGGGGGGGGGGG6480.12572027239392353No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT300.00591875714.0037851
CTCCTGT601.5870683E-714.0037851
CTCCTGG300.00591875714.0037851
GTCCGTG300.00591875714.0037851
GTCCGGT300.00591875714.0037851
TTCCTTT601.5870683E-714.0037851
TTCCTGG601.5870683E-714.0037851
GTCCTTT559.1038237E-714.0037851
CCTCTTT601.5870683E-714.0037851
GGCCTTG300.00591875714.0037851
CCTCTGT652.7726855E-814.0037851
GTCCTGG758.5128704E-1014.0037851
CCTCTGG453.0190005E-514.0037851
GGCCTGT453.0190005E-514.0037851
GCTCGTT453.0190005E-514.0037851
GCTCGGT300.00591875714.0037851
CTGCTTG300.00591875714.0037851
CTTCGGG300.00591875714.0037851
CTGCTGT652.7726855E-814.0037851
TCTCTTT559.1038237E-714.0037851
Produced by FastQC (version 0.11.5)