FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816106
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG19060.10494982121087647No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT1250.014.0086521
CGCCTTT300.005912724414.0086521
TTCCGTG559.104606E-714.0086521
CTCCTGG1250.014.0086521
TTCCGGT453.0173822E-514.0086521
CCTCGTT300.005912724414.0086521
CCTCGTG601.5878322E-714.0086521
CCGCTTT300.005912724414.0086521
GGCCGGT300.005912724414.0086521
TCGCGTT453.0173822E-514.0086521
CCGCTGT601.5878322E-714.0086521
GCGCGTG300.005912724414.0086521
TTCCTTT905.456968E-1214.0086521
TGCCTTT453.0173822E-514.0086521
CTGCGTG453.0173822E-514.0086521
CTGCGGT453.0173822E-514.0086521
CTGCGGG559.104606E-714.0086521
TCTCGTT453.0173822E-514.0086521
TCTCGTG453.0173822E-514.0086521
CCTCTGT1100.014.0086521
Produced by FastQC (version 0.11.5)