FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34526
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT580.16798934136592714No Hit
GGGGGGGGGGGGGGGGGGGG580.16798934136592714No Hit
GGGTTTGGGTTTGGGTTTGG470.13612929386549266No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG452.8436321E-514.0130635
GGTTGTC300.005746755314.01306253
TTGTTCT300.005746755314.01306254
TTGTTCG300.005746755314.01306254
GTGGGCG300.005746755314.01306254
TGGGCTG504.8698003E-614.01306255
TTTGGCG300.005746755314.01306254
TGGGCGT300.005746755314.01306255
TTGGTCT300.005746755314.01306254
TTGTGCG300.005746755314.01306254
GTGTGCT401.663993E-414.01306254
TGTGCTG504.8698003E-614.01306255
TGTGTGC401.663993E-414.01306253
GTTGTTC401.663993E-414.01306253
GTTGTGC300.005746755314.01306253
GTTGTCT300.005746755314.01306254
TGGTCTG558.3534906E-714.0130625
GTGGTCT359.762898E-414.0130624
TGTCTGG359.871089E-413.9927546
TCTGGTT300.005802515413.9927548
Produced by FastQC (version 0.11.5)