FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435513
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT21100.14698578138964954No Hit
GGGGGGGGGGGGGGGGGGGG18720.1304063425409592No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT758.54925E-1014.0060641
TTCCGTT350.001014835114.0060641
CTCCTGT1100.014.0060641
GTCCGTG704.8694346E-914.0060641
GTCCGGT559.119758E-714.0060641
GGCCGGG350.001014835114.0060641
TTCCTTT1350.014.0060641
TTCCTTG704.8694346E-914.0060641
TTCCTGG1650.014.0060641
TGCCTGT704.8694346E-914.0060641
TGCCTGG758.54925E-1014.0060641
CTGCGTG350.001014835114.0060641
GGCCTTG1000.014.0060641
CTGCGGG505.2419473E-614.0060641
GTCCTGT2200.014.0060641
CCTCTGT1400.014.0060641
GGCCTGT1000.014.0060641
GCGCTTT559.119758E-714.0060641
GCTCGGT350.001014835114.0060641
GCGCTGT505.2419473E-614.0060641
Produced by FastQC (version 0.11.5)