FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165242
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG23380.10797869245100548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG1000.014.0118831
TTCCGTG1000.014.0118831
CCGCTGG505.2224113E-614.0118831
GCGCGTT505.2224113E-614.0118831
TGCCTGT1850.014.0118831
CCCCGTG505.2224113E-614.0118831
CTGCGGG505.2224113E-614.0118831
GCGCTGG1000.014.0118831
CTTCGTT1000.014.0118831
CTGCTGG1850.014.0118831
TCCCGTT505.2224113E-614.0118831
CCCCTGT1000.014.0118831
CGTCTTG505.2224113E-614.0118831
CTTCTGG4000.014.0118831
TCCCTGT1000.014.0118831
CGCCTTG801.4915713E-1014.0118821
CTCCTGT1750.014.0118821
CGCCTGT801.4915713E-1014.0118821
CCTCGTT758.4946805E-1014.0118821
GTCCGTG1250.014.0118821
Produced by FastQC (version 0.11.5)