FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809783
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT144830.21267931738794027No Hit
GGGGGGGGGGGGGGGGGGGG112880.16576152279742246No Hit
GGGTTTGGGTTTGGGTTTGG93120.1367444454544293No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTT1200.014.0083261
GTCCGTG6600.014.0083261
TGCCTTG3400.014.0083261
CCTCTTT4800.014.0083261
CCCCGTT1650.014.0083261
CTGCGGT1350.014.0083261
CTGCGGG1350.014.0083261
GCTCGGT2650.014.0083261
CGTCGTT300.005915844314.0083261
CGGCTTG601.5904698E-714.0083261
CCCCTTG6550.014.0083261
CTTCGGT2650.014.0083261
TCTCTTT3950.014.0083261
GCTCTTT3800.014.0083261
CTTCTTT4950.014.0083261
TCCCTGG7700.014.0083261
CTCCTTT5250.014.0083251
CGCCTTT505.240121E-614.0083251
CGCCTTG1150.014.0083251
CTCCTGT10900.014.0083251
Produced by FastQC (version 0.11.5)