FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282394
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT23010.17943003476310712No Hit
GGGGGGGGGGGGGGGGGGGG17970.14012854083846304No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT852.7284841E-1114.0065551
TGCCGTT300.005917342414.0065551
TTCCGGG453.020278E-514.0065551
GTCCGTG601.5895057E-714.0065551
GGCCGTT300.005917342414.0065551
CCTCGGG300.005917342414.0065551
GCGCGTG300.005917342414.0065551
GCGCGGG300.005917342414.0065551
GGCCTGT852.7284841E-1114.0065551
GCTCGTT453.020278E-514.0065551
GCGCTGG852.7284841E-1114.0065551
CTTCGGT453.020278E-514.0065551
TCTCTTG852.7284841E-1114.0065551
CCCCTGT852.7284841E-1114.0065551
TCTCTGG1950.014.0065551
GCCCGTG453.020278E-514.0065551
CTTCTTT1950.014.0065551
CTTCTGG2050.014.0065551
CTCCGGT453.020278E-514.0065551
TCCCTTT601.5895057E-714.0065551
Produced by FastQC (version 0.11.5)