FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016760
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG22660.11235843630377437No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT1350.014.00960351
TTCCGTG401.7445786E-414.00960351
TGCCGTT559.099076E-714.00960351
CTCCTGG2250.014.00960351
CGCCTGT801.4915713E-1014.00960351
TTCCGGT801.4915713E-1014.00960351
CCTCGTT401.7445786E-414.00960351
CCTCGTG401.7445786E-414.00960351
CCGCTTG401.7445786E-414.00960351
GGCCGGT559.099076E-714.00960351
CCGCTGG401.7445786E-414.00960351
TGCCTTG559.099076E-714.00960351
GTCCTTT1750.014.00960351
CTGCGGG401.7445786E-414.00960351
GTCCTGT1750.014.00960351
TCGCTGT758.5128704E-1014.00960351
GCGCTTT801.4915713E-1014.00960351
GCGCTTG801.4915713E-1014.00960351
GCTCGGT559.099076E-714.00960351
GCTCGGG401.7445786E-414.00960351
Produced by FastQC (version 0.11.5)