FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22914
Sequences flagged as poor quality0
Sequence length20
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG1000.4364144191324081No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG300.00570999813.9978165
CTGGGGG300.00570999813.9978169
TTGGGCT300.00570999813.9978164
TGGGCTG401.6406899E-413.9978165
GGGGCGG300.00570999813.9978165
GTCGGTG300.00570999813.9978167
CTGTGGT454.4478098E-412.4425049
TGGGCTT400.00264386712.248095
GGGCGGG400.00264386712.248096
GGGCTGG551.8289592E-411.452766
GGCTGGG500.001078266511.1982547
GCGGGGG450.00637556310.8871918
GTCTGTG450.00637556310.8871917
GGCGGGG450.00637556310.8871917
GCTGGGG550.0023880210.180238