FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524493
Sequences flagged as poor quality0
Sequence length20
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG10810.20610379928807437No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG601.5857586E-714.0048651
CTCCTGT905.456968E-1214.0048651
CTCCTGG1300.014.0048651
CGCCTGG350.001013982814.0048651
GGCCGTG300.005915916514.0048651
CCTCGGT350.001013982814.0048651
GTCCGGG505.2313517E-614.0048651
CCTCGGG453.0170082E-514.0048651
GGCCGGG652.7703209E-814.0048651
TTCCTGG453.0170082E-514.0048651
TGCCTGT350.001013982814.0048651
GTCCTTG601.5857586E-714.0048651
CCTCTTG505.2313517E-614.0048651
GTCCTGT652.7703209E-814.0048651
CCTCTGT453.0170082E-514.0048651
GGCCTGT601.5857586E-714.0048651
GGCCTGG905.456968E-1214.0048651
GCGCTGG453.0170082E-514.0048651
CTGCTTT300.005915916514.0048651
CTGCTGT652.7703209E-814.0048651
Produced by FastQC (version 0.11.5)