FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244359
Sequences flagged as poor quality0
Sequence length20
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG7820.3200209527784939No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT505.1919105E-614.0079861
GTCCTGT1000.014.0079861
CCCCTGG1150.014.0079861
CTCCGGG505.1919105E-614.0079861
TCCCTGG505.1919105E-614.0079861
CTCCTTG350.00100955814.0079851
CTCCTGG1400.014.0079851
CGCCTGG300.00589525914.0079851
TGCCGGG300.00589525914.0079851
GTCCGTT350.00100955814.0079851
GTCCGTG758.367351E-1014.0079851
CCTCGTG350.00100955814.0079851
GGCCGTG350.00100955814.0079851
GTCCGGG452.9977362E-514.0079851
CCTCGGG350.00100955814.0079851
GGCCGGG758.367351E-1014.0079851
TTCCTGG559.016894E-714.0079851
TGCCTGT452.9977362E-514.0079851
TGCCTGG452.9977362E-514.0079851
CCTCTTT350.00100955814.0079851
Produced by FastQC (version 0.11.5)