FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25217
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT470.1863822024824523No Hit
GGGGGGGGGGGGGGGGGGGG330.13086409961533885No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT359.702937E-414.06
TTTTCTG452.8084865E-514.05
TTTTCGG300.005725334414.05
TCTGTGT300.005725334414.08
TTTTGCT401.6489839E-413.9999994
CTGGGTG551.282449E-512.7272739
TTGCTGG400.002653637412.2499996
Produced by FastQC (version 0.11.5)