FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658977
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG14250.21624426952685755No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGG652.7650458E-814.0085051
GTCCTTT652.7650458E-814.0085051
GTCCTTG652.7650458E-814.0085051
CCTCTTG652.7650458E-814.0085051
GTCCTGT1300.014.0085051
CTGCTTG652.7650458E-814.0085051
CCCCTGG1250.014.0085051
CTTCTGT1150.014.0085051
TCCCTGT652.7650458E-814.0085051
TCCCTGG1250.014.0085051
GCCCTGG1750.014.0085051
CTCCTTT350.001012476714.0085041
CTCCTTG801.4915713E-1014.0085041
TTCCGTG559.0803405E-714.0085041
CTCCTGT852.5465852E-1114.0085041
CTCCTGG1450.014.0085041
GTCCGTT350.001012476714.0085041
GTCCGTG905.456968E-1214.0085041
CCTCGTG559.0803405E-714.0085041
GGCCGTG505.222184E-614.0085041
Produced by FastQC (version 0.11.5)