FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length20
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGGGCGGGGGTGGTGG12.0No Hit
TTGTTTTTCTGGTGGGTGGG12.0No Hit
GTCGGGGGTGGGGTGTGGGG12.0No Hit
GTGGTGGGCGGGTGGTGGTG12.0No Hit
TCCCTGGGCTGTGGTGGGGT12.0No Hit
TGTGTTGGGGTTGTGGTGGT12.0No Hit
GTGTTTTGATGGGGGGGGGG12.0No Hit
GTTTGTGGGGGTTTGGTTGG12.0No Hit
GTTCTTTTGTGGTGTTTTTT12.0No Hit
GTGGTTGTGTTGGTGTTTGG12.0No Hit
GGTTTGTTTTGTTTGTTGTT12.0No Hit
CTGGTGTGGTGTTGTGGGTG12.0No Hit
GTTGTGGTGGGTGGTTTTGG12.0No Hit
TTGTTGTGGGTGTGGTGGGT12.0No Hit
GGGGGGGGGTGGGGGGGGTG12.0No Hit
GTGTGGGTCTGTGTGTGTGT12.0No Hit
GGTTTTGTGTGTGTTGGTTT12.0No Hit
GGTTTGGTGTGGGTGGGGTT12.0No Hit
GTTCTTGTCGTTGGTGGGGT12.0No Hit
GTTTTGTGGTGTTGTGTGGT12.0No Hit
CTTGGTTTTTGGTGGTTGTG12.0No Hit
CGTGTTGTTTTGTGGGTGGT12.0No Hit
TTTGTTGGGTGGGGGGGGGG12.0No Hit
CGTTTTGTTGGTTGGGGTTT12.0No Hit
GGTGGGTGGTGGGGGGTGGG12.0No Hit
AGGTGTGTGTGTGGGTGGTG12.0No Hit
GGTCTTTTGTGGTTGTGTGG12.0No Hit
CGGGTTGGTGTTGTTGGGTG12.0No Hit
GGGTGTTTGTTTTTTGGGTT12.0No Hit
TTGGTTTTGGGTTTTGTGTG12.0No Hit
GGTTTTGTCGTTGTGTTTTT12.0No Hit
GGGGTGGGCGTTGGTTGGTG12.0No Hit
TTCTTTTGGTTTGTGGGTGT12.0No Hit
GGTTGGGGGGGGTGTTTTGT12.0No Hit
GTTTGGTTTGGGTGTGGTTG12.0No Hit
AGTGTTGGGGGGGTGTGGTT12.0No Hit
GTTTTTTTGGTTGGTTTTTG12.0No Hit
GTGGGTGGTGTTTTTTGGTG12.0No Hit
GTTCTTGTGTGTGTGTTGGG12.0No Hit
TGTTTTGTTTGTGGTGTGGG12.0No Hit
GGGGGGGTGGGGGGGGGGGG12.0No Hit
CTCTTGGTCGGGGGGGTGTG12.0No Hit
GGGGGGGTGGGTGGTGTGTT12.0No Hit
TGGTTGTGTTGTGGGTTGTG12.0No Hit
GGCGGGTGAGGTTGTTTGGG12.0No Hit
GCCTTGTTGTGGGGGGGTTG12.0No Hit
CTGTTGGTCTGTGTGTTGTG12.0No Hit
GGGGTGGGGGGTGGGGGGGG12.0No Hit
GCTTGTTGTGGGGTGTTGTT12.0No Hit
GGGGTTGGGTTGGGTTTGTG12.0No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG50.013.9999998
GGGGGTG50.013.9999993
GTGGGGG50.013.9999997
GGGGGGT50.013.9999992
GGTGGGG50.013.9999996
GGGGTGG50.013.9999994
GGGTGGG50.013.9999995
Produced by FastQC (version 0.11.5)