FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGGTTATTTTTGTTTGT11.1764705882352942No Hit
GGGGTTGGGGTTTGGTTGTT11.1764705882352942No Hit
GGTCTGTGGGGTTTGGGGTG11.1764705882352942No Hit
CGTTGTTTTTGTGTTGGGTG11.1764705882352942No Hit
ATTTTGTTGGTTTGTTTTTT11.1764705882352942No Hit
TGTTTGTTGTTGTGTTTTTT11.1764705882352942No Hit
GTTTTGTGGGGGTGTTGGGT11.1764705882352942No Hit
GGTTTTTGCTGTGGTTTGTG11.1764705882352942No Hit
TGGGTTGGCTGTTGGGGGGG11.1764705882352942No Hit
GTTTGTTTCTTGTTGTGTTT11.1764705882352942No Hit
GGTGTTGGGGGGTGGTTTGT11.1764705882352942No Hit
GGTGTTGGCGGTGTGTTTGT11.1764705882352942No Hit
TCCTGTTGCGGGTTTGGGTG11.1764705882352942No Hit
GTGTTTGGGTTTGGGGTGTT11.1764705882352942No Hit
GGGGTTTTCTTGGGTGTTTT11.1764705882352942No Hit
GTGGTGGGGGTGGTTTTGGT11.1764705882352942No Hit
TGTTGTGGCTGGTGTGGGTT11.1764705882352942No Hit
ATTTTTTTGTGTGGGTTGTT11.1764705882352942No Hit
GGTGTTTGTTGTTGTTTGTG11.1764705882352942No Hit
TGTTTTTGTGGGGGTTTTGG11.1764705882352942No Hit
TTTTTGGGCTTTGGTTTTTT11.1764705882352942No Hit
GTGGTTGTGTGTGGGGTGGT11.1764705882352942No Hit
GTTTTGGGTTTTGGGTTTTG11.1764705882352942No Hit
GTTGGTTGCTTTTGGTTTTG11.1764705882352942No Hit
GTTGTTTTGTGGGTGTTGGT11.1764705882352942No Hit
GTTTGGTTCTGTTTTGGTTG11.1764705882352942No Hit
GTTTTTTTGGGTGTTTTTGG11.1764705882352942No Hit
ATTTTTTGCGGTGGGGTTTT11.1764705882352942No Hit
GTGGGGTTTTTTGGGTTTTG11.1764705882352942No Hit
GTGTTTTGTTTGGGGGTTTT11.1764705882352942No Hit
CTTTTTTGCTGTGGTTGTGT11.1764705882352942No Hit
GTGTGGTTGTGGGGTTGGTG11.1764705882352942No Hit
GGGGTGGTCGGGGGTGGGGT11.1764705882352942No Hit
GGGGTGGTTGGTTGGTTTGT11.1764705882352942No Hit
GTTTTTTTCTTTTTTTGTTT11.1764705882352942No Hit
CGTGTGTTGTGTGTTGTTTG11.1764705882352942No Hit
CGTGGTTGCTTGGTTTGTTT11.1764705882352942No Hit
GGGTGGGTGTGGTGGGTGTT11.1764705882352942No Hit
GTGCGGGTGGGTGGTGGTTG11.1764705882352942No Hit
CTGGTTTTCGTTGTGGGTTT11.1764705882352942No Hit
GTTTTTTGCGGTGTTTTTGT11.1764705882352942No Hit
GGGGTTGGGTTTTTTGGGTT11.1764705882352942No Hit
CGTTTTTGGGGTTGTGGTGT11.1764705882352942No Hit
CGTGTGTTATTTTTTTTTGT11.1764705882352942No Hit
GTTGTGGTGTTTGTTGTTGT11.1764705882352942No Hit
TTGGGTTGCGGGTTGTGGGT11.1764705882352942No Hit
GGTGGTTGCGGGGTGGGTTG11.1764705882352942No Hit
TTGTGTTTCTTGGGTGTTGT11.1764705882352942No Hit
GTTTTTGTCTTTTGGTGGTG11.1764705882352942No Hit
GTGCTGGGGGGTGGGGGGTT11.1764705882352942No Hit
ATTTGTGTCTTGTTGGTTTT11.1764705882352942No Hit
GGTTGGGGCGGTTTGTGGTT11.1764705882352942No Hit
TTGGTTTTTTTTTGGTTTTT11.1764705882352942No Hit
GGTTGTGGTGTGGTGGGTTT11.1764705882352942No Hit
CTTTTGTCTGTGGGTGGTGT11.1764705882352942No Hit
GTTGTGTGGTGTGGGGGGTG11.1764705882352942No Hit
TCTTGTTTATTGGGTTGGGT11.1764705882352942No Hit
GTGGGTTTATTGGTGTGGGG11.1764705882352942No Hit
TTGGGTTTATAATGTTGTTT11.1764705882352942No Hit
GGGGGGTTGGTGTTGGGTGG11.1764705882352942No Hit
GGGGGGGTGTGGGGTTGGGT11.1764705882352942No Hit
GGTTTTTTTTTTGTTTTTTT11.1764705882352942No Hit
GGTTGGGGCGTTTTTTGTTT11.1764705882352942No Hit
TTGTTGGGTGTGTTTGGTTG11.1764705882352942No Hit
TGGTTGGGGTTGTGGTTTTT11.1764705882352942No Hit
GGGGTTGTGTGTGGGGTTGG11.1764705882352942No Hit
GGGGTTGTCTGGGGGGGTTT11.1764705882352942No Hit
CGTTGGTGGGGGGGTTGTTG11.1764705882352942No Hit
TGGCTGGTGTTTTTGTTGGG11.1764705882352942No Hit
AGGGTGTTGTTGGTGGTTTT11.1764705882352942No Hit
TGGCTTTGGTGTTGTGTGGT11.1764705882352942No Hit
GGTTTTTTGTTTTGGGGTTT11.1764705882352942No Hit
AGTTTGTTGGTTTTGGGTGG11.1764705882352942No Hit
GGGTGGTTGTGTGTGTTGTT11.1764705882352942No Hit
GGGTGTTTTTGTGGGTGGGT11.1764705882352942No Hit
GTGTTTGGGGTTGTGGTTGG11.1764705882352942No Hit
GTGTTTTTGGGGTGGGTTTT11.1764705882352942No Hit
GGCTTGTTCTTTGTTTTTGT11.1764705882352942No Hit
TTGTTGTTGTGTTTTTTTTG11.1764705882352942No Hit
GGCTGTGGCGGTTGTGTTTG11.1764705882352942No Hit
TGTTTGGTGGGGTTGGGTTT11.1764705882352942No Hit
GGTTTGGGGGTTTTTTGTGT11.1764705882352942No Hit
GTGGTGGGGTGTGGTTTTGT11.1764705882352942No Hit
CTTTGTTGGGGTTTTGGTTT11.1764705882352942No Hit
GTGTTGGTATTGTGTGTGTG11.1764705882352942No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGTG50.013.9999998
GTGGTGG50.013.9999999
TGGTGGG50.013.99999910
GGGTGTT50.013.99999914
GGGTGTG50.013.9999995
GTGTGGT50.013.9999997
GGGTGGG50.013.9999991
GGTGTGG50.013.9999996
GTGGGTG100.06.99999953
TGGGTGT100.06.99999954
GGTGGGT100.06.99999952