FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259010
Sequences flagged as poor quality0
Sequence length20
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3830.14787073858152194No Hit
GTGTGTGTGTGTGTGTGTGT3700.14285162735029536No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT401.7385314E-414.0065681
CTCCTTG401.7385314E-414.0065681
GTCCGTG453.0019666E-514.0065681
GGCCGTG300.005900479414.0065681
GGCCGGG300.005900479414.0065681
TTCCTTG453.0019666E-514.0065681
TGCCTTG300.005900479414.0065681
TGCCTGG453.0019666E-514.0065681
GTCCTTG903.6379788E-1214.0065681
CCTCTTG601.5728983E-714.0065681
GGCCTTG401.7385314E-414.0065681
GTCCTGT758.403731E-1014.0065681
GTCCTGG453.0019666E-514.0065681
GGCCTGT300.005900479414.0065681
CCCCTTG453.0019666E-514.0065681
CTGCTGG453.0019666E-514.0065681
TCTCTTG601.5728983E-714.0065681
CCCCTGT601.5728983E-714.0065681
GCTCTTG300.005900479414.0065681
GCCCGTG453.0019666E-514.0065681
Produced by FastQC (version 0.11.5)