FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189022
Sequences flagged as poor quality0
Sequence length20
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT4580.24229983811408196No Hit
GGGTTTGGGTTTGGGTTTGG3070.16241495698913352No Hit
GGGGGGGGGGGGGGGGGGGG2300.12167895800488833No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTTG505.19439E-614.003441
GTCCTGT300.005901418614.003441
GTCCTGG452.999826E-514.003441
GCTCTTG350.001010548714.003441
GCTCTGG300.005901418614.003441
CTTCTTG300.005901418614.003441
TTTGTTC1050.013.9997373
TTTGTCG1050.013.9997374
TTTTGTC1200.013.9997363
TGTTGTC652.7464921E-813.9997363
CTCTGGT300.005911784713.9997362
TTGTCTG2050.013.9997365
TGGTTTC453.0073204E-513.9997363
GTATGTG505.2086507E-613.9997367
GGATGTT300.005911784713.9997367
TGTTGGA300.005911784713.9997363
TTTTGCT1450.013.9997364
TTGTCGG758.403731E-1013.9997365
TTTTGAT300.005911784713.9997364
TTTTGAG453.0073204E-513.9997364
Produced by FastQC (version 0.11.5)