FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160025
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT3430.214341509139197No Hit
GGGGGGGGGGGGGGGGGGGG2320.14497734728948602No Hit
GGGTTTGGGTTTGGGTTTGG2220.13872832369942195No Hit
GTCCTTTTGTGTGTTTTTGT1840.11498203405717856No Hit
GTCCTTGTGTGGTGTTTTGT1620.10123418215903764No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG401.7260744E-414.0121991
CTCCTGT558.930474E-714.0121991
TTCCTGT558.930474E-714.0121991
TTCCTGG350.001004591414.0121991
GTCCTTT558.930474E-714.0121991
GTCCTGG801.4370016E-1014.0121991
CTGCTGT350.001004591414.0121991
TCTCTGG350.001004591414.0121991
GCTCTGT350.001004591414.0121991
CTTCTTT350.001004591414.0121991
CTCTTGT300.005871780714.0121981
TTCCTTT452.9765471E-514.0121981
GTCCTTG505.1489205E-614.0121981
GGCCTTG300.005871780714.0121981
CCTCTGT300.005871780714.0121981
CCTCTGG300.005871780714.0121981
CCCCTGG300.005871780714.0121981
TCTCTGT452.9765471E-514.0121981
GCTCTTT452.9765471E-514.0121981
CTTCTGT452.9765471E-514.0121981
Produced by FastQC (version 0.11.5)