FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTGGTGTGTTGTTTT11.5384615384615385No Hit
GGGGTGGGGGGGTTTGTGGT11.5384615384615385No Hit
GTTTGTTGGTTGGGGGGTTT11.5384615384615385No Hit
CGTTTTTGGTTGGTGTTTTG11.5384615384615385No Hit
CGTGTTTTCTGTGGTTTGTG11.5384615384615385No Hit
GTTCTTTGTTTTGGTTTTTG11.5384615384615385No Hit
CTGGTGGGGTGTGGTGTGTG11.5384615384615385No Hit
AGTGGGGGGTGTGTTGGGTT11.5384615384615385No Hit
GTGTTTTTGGTGTTTTTTTG11.5384615384615385No Hit
GCTGTTTTGGGGTGGTGTGT11.5384615384615385No Hit
TGTCTTGTCTGTTGTTTTTG11.5384615384615385No Hit
CTGGGTGGGTGGGTGGGTTT11.5384615384615385No Hit
GCTTGGGTTGTTGGTTGGGT11.5384615384615385No Hit
GTCCGTGGGTGGTTGTGTGG11.5384615384615385No Hit
GGGGTGGGGTGGTGGTGTGG11.5384615384615385No Hit
GGGGTTTTGTTGGGGTGGGG11.5384615384615385No Hit
GTGGTGGGCGTGGGGTTTTG11.5384615384615385No Hit
TGTGGTTGCTGTTTTGTTGG11.5384615384615385No Hit
CGGTTTTGGGTTGGTGGTTG11.5384615384615385No Hit
GGTGTGTTGTGGTGGTGTGT11.5384615384615385No Hit
GGTTGGTTTTGGTGGTGGGG11.5384615384615385No Hit
CGGTTTTGGTGTTTTGGGGT11.5384615384615385No Hit
GGTGTTTGTTTTGGTTTGTG11.5384615384615385No Hit
GTGTTTGTGTGTTGGGGTGG11.5384615384615385No Hit
TGGTGTTTCTGTGGGGTTGG11.5384615384615385No Hit
GTTGTTGTGGGTGTTGTGTG11.5384615384615385No Hit
GGTGTGGGGTGGTGTGGTTT11.5384615384615385No Hit
GGGTTGTTGTTTGGGTTTGG11.5384615384615385No Hit
CGGTTTTTGGGGTGGTGGTT11.5384615384615385No Hit
TTCCGTTTGTGGGGTGGTGT11.5384615384615385No Hit
GTTGTTTTGTTTTGGTTTGT11.5384615384615385No Hit
CGTGTGTGGTGTGGGTGTGG11.5384615384615385No Hit
TCTCTGTGGTGTTGTGTGTG11.5384615384615385No Hit
GTCTTTGGTTGTTTTGTTTG11.5384615384615385No Hit
GCTTGTGTTTGTGGGTGTTG11.5384615384615385No Hit
TTTCTGGTGTGGTGTGGTTT11.5384615384615385No Hit
TGGTTGTGGGGTGTGGTTTT11.5384615384615385No Hit
GGGTGTGTGGGTGGGGTGGT11.5384615384615385No Hit
GGTGTTGTTTGGTGTTTTTG11.5384615384615385No Hit
CCCTTTGTGGTTTGGTTGTG11.5384615384615385No Hit
TTTGTTGTGTTTGGTTTTTG11.5384615384615385No Hit
GTTCGGTTGTTTGTTTGTGG11.5384615384615385No Hit
GTCGTTGTGTGTTGGGGTGT11.5384615384615385No Hit
GGTGTGTTCGTTTGTGTTGT11.5384615384615385No Hit
GGTGTTTTTTGTTTGGGTTT11.5384615384615385No Hit
GTTTTTTTGGGTTTTGTGTG11.5384615384615385No Hit
GTGGTGGTGGTGGTGTTTGT11.5384615384615385No Hit
GTGGTTGGGGGTGGTTTGTT11.5384615384615385No Hit
CCCTGGGGTGTGTGGTGGTG11.5384615384615385No Hit
TGGTTGGTGTGGTTTGGTTT11.5384615384615385No Hit
CGTTTGGTGTGGTGGTTTGG11.5384615384615385No Hit
GTCCTGTGTGTTTGGTTTGG11.5384615384615385No Hit
TTCCGTTTCGGTTTGGGTTT11.5384615384615385No Hit
GGTGTGTGGGTTGGTGGTGG11.5384615384615385No Hit
CCGTTTTGGTGGGTGGGGTG11.5384615384615385No Hit
GGGTTTTTATGTTTTGTTTT11.5384615384615385No Hit
TTGTGGTTGTGGGGTGTGGT11.5384615384615385No Hit
GTGTTTGTGTTTGTTGTGGT11.5384615384615385No Hit
GGGTGTGGGTGTGGGTGGTG11.5384615384615385No Hit
TTCCTTGTCTTTTGTGTGTT11.5384615384615385No Hit
GGTTTGGTGGGGGTGTTGTG11.5384615384615385No Hit
GGTTTGTGGTGGTTGGTGGT11.5384615384615385No Hit
TCCTTTGGCGGTGGTTGGTG11.5384615384615385No Hit
CTTGTGGGCTGTGGTGTTTT11.5384615384615385No Hit
GTTGTTTGTTGGGTGTTGGG11.5384615384615385No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTG50.013.9999991
TGGTTGG50.013.99999912
GGTTGGT50.013.99999913
GCGGTGG50.013.9999998
CGGTGGT50.013.9999999
CCTTTGG50.013.9999992
TTTGGCG50.013.9999994
TTGGCGG50.013.9999995
GTTGGTG50.013.99999914
GGTGGTT50.013.99999910
GTGGTTG50.013.99999911
TGGCGGT50.013.9999996
CTTTGGC50.013.9999993
GGCGGTG50.013.9999997