FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235350
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT4800.20395156150414276No Hit
GGGGGGGGGGGGGGGGGGGG3330.14149139579349906No Hit
GGGTTTGGGTTTGGGTTTGG2860.12152113872955174No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG300.00588702514.01063251
TTCCTGG300.00588702514.01063251
GGCCTGG300.00588702514.01063251
TCCCTGT300.00588702514.01063251
TTCCTGT452.9914774E-514.0106321
TGCCTGG350.001007922314.0106321
GTCCTTG350.001007922314.0106321
GTCCTGG350.001007922314.0106321
CCTCTGG350.001007922314.0106321
CTGCTGG401.7331887E-414.0106321
TCTCTGG350.001007922314.0106321
GCTCTTG401.7331887E-414.0106321
GCTCTGT452.9914774E-514.0106321
GCTCTGG350.001007922314.0106321
CTTCTTT350.001007922314.0106321
CTTCTTG401.7331887E-414.0106321
CTTCTGT350.001007922314.0106321
TTTGCTG1750.013.9987275
CTTTTGC300.005920310513.9987263
TGGTTTC300.005920310513.9987263
Produced by FastQC (version 0.11.5)