FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33595
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG510.15180830480726298No Hit
GTGTGTGTGTGTGTGTGTGT440.13097187081410924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTG300.005741351314.0134335
TTGTTCT300.005741351314.0134334
CCTGTGT300.005741351314.0134332
TGGGCTT300.005741351314.0134335
TGTGGCT300.005741351314.0134334
GTGTCTG359.750995E-414.01343255
TGTTCTG359.750995E-414.01343255
TGTGTCT401.6614409E-414.01343254
GTATGTG359.862152E-413.9925497
TATGTGG300.00579865313.9925498
TCGTTGG300.00579865313.9925498
TGTATGT300.00579865313.9925496
TGTCGTT359.862152E-413.9925496
GTCGTTG401.682744E-413.9925487
TGTCTGT454.5600085E-412.437826
TCTGTGT450.006500140810.8830938
Produced by FastQC (version 0.11.5)