FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501471
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG12050.24029305782388216No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT505.204365E-614.0115781
TTCCTGT401.7383764E-414.0115781
CCTCTGT758.440111E-1014.0115781
GGCCTGG801.4733814E-1014.0115781
CTGCTTG758.440111E-1014.0115781
CCCCTTG401.7383764E-414.0115781
CTGCTGG505.204365E-614.0115781
TCTCTGT401.7383764E-414.0115781
CTTCTTT505.204365E-614.0115781
CTTCTGG1000.014.0115781
CTCCGGG401.7383764E-414.0115781
GCCCTTT401.7383764E-414.0115781
GCCCTGG505.204365E-614.0115781
CTCCTTT601.576027E-714.0115771
CTCCTTG852.5465852E-1114.0115771
CTCCTGG559.0454523E-714.0115771
GTCCGTT350.001010206914.0115771
TTCCTTT652.751949E-814.0115771
TTCCTTG559.0454523E-714.0115771
TTCCTGG300.005896675414.0115771
Produced by FastQC (version 0.11.5)