FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9542465
Sequences flagged as poor quality0
Sequence length20
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG188140.19716079650279042No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTT758.54925E-1014.0089571
TTCCTTG6150.014.0089571
TGCCTTT2000.014.0089571
CCTCTTG9450.014.0089571
TCTCGTT2000.014.0089571
TCGCTTT758.54925E-1014.0089571
CAGTTTG350.001013989614.0089561
CTCCTGT14650.014.0089561
TTCCGGT1900.014.0089561
CCTCTTT5350.014.0089561
CGGCTTT350.001013989614.0089561
TCCCGTG3150.014.0089561
TCCCGGT2550.014.0089561
CGTCTTT905.456968E-1214.0089561
CGTCTTG1150.014.0089561
TCCCTTG5700.014.0089561
CTCCTTT5900.014.0089551
TTCCGGG2150.014.0089551
TCGCGTG401.7463401E-414.0089551
CGCCGTT601.5898331E-714.0089551
Produced by FastQC (version 0.11.5)