FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216462
Sequences flagged as poor quality0
Sequence length20
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG189760.36377146042662634No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG5800.014.0083551
CCTCGTG5400.014.0083551
CCTCGGT3450.014.0083551
TGCCTTT1350.014.0083551
TCGCTGG1350.014.0083551
GCTCGGT2700.014.0083551
CTTCGTT1450.014.0083551
CTTCGTG2900.014.0083551
CGTCGTG652.7801434E-814.0083551
CTGCTTG2700.014.0083551
CTTCTTT2600.014.0083551
CGCCGTG1450.014.0083551
CGTCTGG2600.014.0083551
CTCCTTT2550.014.0083541
CGCCTTT758.54925E-1014.0083541
CGCCTTG1550.014.0083541
TTCCGTT801.5097612E-1014.0083541
TTCCGTG1700.014.0083541
TGCCGTT559.116211E-714.0083541
CTCCTGT5750.014.0083541
Produced by FastQC (version 0.11.5)