FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477984
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG10660.2230200174064404No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGT559.0578214E-714.0095281
CTCCTGG704.822141E-914.0095281
CCTCGTG350.001011202614.0095281
CCTCGGT350.001011202614.0095281
TTCCTTT401.7402667E-414.0095281
TTCCTGG559.0578214E-714.0095281
TGCCTGT401.7402667E-414.0095281
TGCCTGG350.001011202614.0095281
GTCCTTG350.001011202614.0095281
GGCCTTG350.001011202614.0095281
GTCCTGT559.0578214E-714.0095281
CCTCTGT350.001011202614.0095281
GTCCTGG559.0578214E-714.0095281
CCTCTGG350.001011202614.0095281
GGCCTGT350.001011202614.0095281
CTTCGTG559.0578214E-714.0095281
CTGCTTT401.7402667E-414.0095281
CTGCTGG401.7402667E-414.0095281
TCTCTTT350.001011202614.0095281
CCCCTGG401.7402667E-414.0095281
Produced by FastQC (version 0.11.5)