FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297626
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG7190.24157835672958683No Hit
CTGTGTGTTTTTGTGTTGTG5910.1985713613729983No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG559.015748E-714.0107641
GTCCTGT350.00100895314.0107641
GGCCTGG559.015748E-714.0107641
CTGCTGG350.00100895314.0107641
CCCCTGG350.00100895314.0107641
GCTCTGT559.015748E-714.0107641
GCTCTGG559.015748E-714.0107641
TCCCTGT350.00100895314.0107641
TCCCTGG704.78758E-914.0107641
CTCCTGT452.9964573E-514.0107631
GGCCGTG401.7354844E-414.0107631
TTCCTGG401.7354844E-414.0107631
GTCCTGG452.9964573E-514.0107631
CCTCTGG401.7354844E-414.0107631
GGCCTGT401.7354844E-414.0107631
GCTCGTG300.00589149714.0107631
CTGCTTT300.00589149714.0107631
TCTCTTT300.00589149714.0107631
TCTCTGT452.9964573E-514.0107631
TCTCTGG300.00589149714.0107631
Produced by FastQC (version 0.11.5)