FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13179210
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG311160.23609912885521972No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTT3200.014.0077991
CCGCGGT1600.014.0077991
CTCCTTT7650.014.0077981
CGCCTTT1100.014.0077981
TTCCGTG4350.014.0077981
CGCCTGT3600.014.0077981
CCGCTTT905.456968E-1214.0077981
CCTCGGG12700.014.0077981
TCGCGGT905.456968E-1214.0077981
TTCCTTT5850.014.0077981
TCGCTTT852.7284841E-1114.0077981
TCCCGTG6000.014.0077981
GCCCGTG10950.014.0077981
CTCCGTT4450.014.0077981
CGCCGTT758.54925E-1014.0077981
CGTCTGT3350.014.0077981
CTCCGGT6800.014.0077981
TCCCTGT13750.014.0077981
CCGCGTT1100.014.0077981
CTGAGTG300.005917709314.0077971
Produced by FastQC (version 0.11.5)