FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9769875
Sequences flagged as poor quality0
Sequence length20
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG287340.29410816412697194No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT5100.014.0088251
CCTCGGG12600.014.0088251
TCGCGTT559.1151014E-714.0088251
CCCCGTT3150.014.0088251
CCTCTGT12250.014.0088251
CGTCGTT559.1151014E-714.0088251
CTGCTTT3450.014.0088251
CGTCGGG2200.014.0088251
TCCCGTG5600.014.0088251
TCCCGGT4650.014.0088251
CTCCGTG12150.014.0088251
CGCCGTG2200.014.0088251
CTCCGGT7400.014.0088251
TCCCTTT3650.014.0088251
GCCCTGT14050.014.0088251
GCCCTGG31400.014.0088251
CGCCTTT1050.014.0088241
TGCCGTT1150.014.0088241
CTCCTGT12850.014.0088241
CGCCTGT3100.014.0088241
Produced by FastQC (version 0.11.5)