FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123396
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG25880.2303729050130141No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG950.014.00828551
TTCCGTT350.00101337814.00828551
CCTCGTT505.230917E-614.00828551
CCTCGGG505.230917E-614.00828551
GGCCGGT350.00101337814.00828551
TGCCTTT505.230917E-614.00828551
CTGCGTG505.230917E-614.00828551
TCTCGTG350.00101337814.00828551
GTCCTGG2050.014.00828551
GGCCTGT852.5465852E-1114.00828551
CCCCGGG350.00101337814.00828551
CTTCGTT505.230917E-614.00828551
CTGCTGT852.5465852E-1114.00828551
CTGCTGG950.014.00828551
CCCCTGT704.8548827E-914.00828551
TCTCTGT1000.014.00828551
TCCCGGG350.00101337814.00828551
GCTCTTG852.5465852E-1114.00828551
GCCCGTG505.230917E-614.00828551
GCCCGGT350.00101337814.00828551
Produced by FastQC (version 0.11.5)