FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210422
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG13790.6553497257891285No Hit
CTGTGTGTTTTTGTGTTGTG8380.39824733155278436No Hit
GTGTGTGTTTTTGTGTTGTG3640.17298571442149585No Hit
GTGTGTGTGTGTGTGTGTGT2160.10265086350286567No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTGT452.991714E-514.0090381
GCTCTGG452.991714E-514.0090381
CTCCTTG300.00588872714.0090371
CTCCTGG300.00588872714.0090371
GTCCTTG300.00588872714.0090371
CCTCTGT350.001008165214.0090371
GTCCTGG300.00588872714.0090371
CCTCTGG350.001008165214.0090371
GGCCTGG300.00588872714.0090371
CTGCTGG601.564731E-714.0090371
TCTCTGT300.00588872714.0090371
CTCCGTT350.001008165214.0090371
CTTCTGT601.564731E-714.0090371
CTTCTGG505.1796305E-614.0090371
TCCCTGT350.001008165214.0090371
TCCTGGG559.0405047E-714.00237752
TTTTGTC1000.013.999053
TGTTGTC652.7541319E-813.999053
GATGGGG300.00591664713.999058
TTGTCTT1100.013.999055
Produced by FastQC (version 0.11.5)