FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13163875
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG288610.21924395362307833No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTT1550.014.00869751
CCTCGGT7650.014.00869751
CCCCGTG7950.014.00869751
CCTCTGT18500.014.00869751
GGCCTGT17250.014.00869751
CGTCGGT1050.014.00869751
TCCCGGT4400.014.00869751
GCTCTTT8100.014.00869751
GCTCTGG28850.014.00869751
CGTCTTG2450.014.00869751
CTCCGTG12950.014.00869751
CTCCGGG12000.014.00869751
CGCCGGT1800.014.00869751
CCGCGTT905.456968E-1214.00869751
GCCCTTT5350.014.00869751
CCGCGGG3500.014.00869751
CTCCTTT7600.014.0086971
CTCCTTG14600.014.0086971
TTCCGTG5200.014.0086971
CGCCTGT3350.014.0086971
Produced by FastQC (version 0.11.5)