FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725548
Sequences flagged as poor quality0
Sequence length20
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG23720.3269253033569109No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT300.005907322714.0089671
CTCCTGT950.014.0089671
CGCCTGG300.005907322714.0089671
TGCCGGG300.005907322714.0089671
CCTCGGT300.005907322714.0089671
GGCCGGT704.840331E-914.0089671
GGCCGGG950.014.0089671
GCGCGGT350.001012397114.0089671
TTCCTGT300.005907322714.0089671
GTCCTTG350.001012397114.0089671
CCTCTTG601.5829391E-714.0089671
GGCCTTT350.001012397114.0089671
CCTCTGT601.5829391E-714.0089671
GGCCTGG2250.014.0089671
CCCCGGG601.5829391E-714.0089671
GCTCGTT350.001012397114.0089671
GCTCGGT758.4946805E-1014.0089671
GCGCTGT704.840331E-914.0089671
GCGCTGG950.014.0089671
CTTCGTT300.005907322714.0089671
Produced by FastQC (version 0.11.5)