FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989053
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG23600.2386120865110363No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTT758.4946805E-1014.0106771
TGCCTGG758.4946805E-1014.0106771
CTTCGTG758.4946805E-1014.0106771
CCCCTTG758.4946805E-1014.0106771
CTCCTTG350.00101192114.0106761
CTCCTGT1100.014.0106761
CTCCTGG1400.014.0106761
CGCCTGT350.00101192114.0106761
GTCCGGT505.220165E-614.0106761
GGCCGGG350.00101192114.0106761
CCGCTGT350.00101192114.0106761
TGCCTTG350.00101192114.0106761
TTCCTGT1350.014.0106761
GTCCTTG704.840331E-914.0106761
GGCCTTT505.220165E-614.0106761
TCTCGTG505.220165E-614.0106761
CCTCTGT559.077721E-714.0106761
GGCCTGG1000.014.0106761
GCTCGTG350.00101192114.0106761
GCGCTGG505.220165E-614.0106761
Produced by FastQC (version 0.11.5)