FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177865
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG4440.24962752649481346No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTGT350.001010018214.0036561
GCTCTTT350.001010018214.0036561
GCCCTGG452.9974284E-514.0036551
TTTTGTC1450.013.999723
TGTTGTC505.204558E-613.999723
TGATGGG350.001012169213.999727
TTGTCTT1150.013.999725
GCGGGGG1150.013.999728
GGATGTT350.001012169213.999727
TTTTGGC704.791218E-913.999723
TTTTGGA505.204558E-613.999723
TTTTGCT704.791218E-913.999724
TGTTGCT350.001012169213.999724
TGTTGCG505.204558E-613.999724
TTTTGAG505.204558E-613.999724
GTTTGTC1000.013.999723
GTGTTTC350.001012169213.999723
GTTTGGC704.791218E-913.999723
GTGTCGG505.204558E-613.999725
GTGTTCT1150.013.999724
Produced by FastQC (version 0.11.5)