FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10566427
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG230120.21778411945684192No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTT3950.014.009421
TCGCTTG905.456968E-1214.009421
GCTCGTT3950.014.009421
CGTCGTT453.0185343E-514.009421
TCCCGGT2900.014.009421
GCCCGGT6450.014.009421
TCCCTTT3600.014.009421
CCGCGTT905.456968E-1214.009421
CCGCGTG1800.014.009421
GCCTCTG950.014.0094191
GCCTCAG505.236543E-614.0094191
CTCCTTT7450.014.0094191
CGCCTTG2200.014.0094191
TTCCGTT1200.014.0094191
TGCCGTT559.110954E-714.0094191
TTCCGGT2050.014.0094191
TTCCGGG2800.014.0094191
GTCCGGT6700.014.0094191
TCGCGTG1000.014.0094191
CCGCTGT2200.014.0094191
Produced by FastQC (version 0.11.5)