FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8161662
Sequences flagged as poor quality0
Sequence length20
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG255980.3136370998946048No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT350.001013913414.0090561
TTCCTGG7700.014.0090561
GTCCTTT4950.014.0090561
GGCCTTT3750.014.0090561
GCGCTTT950.014.0090561
CGTCGTT601.5895966E-714.0090561
CGTCGTG1200.014.0090561
CTTCGGT3600.014.0090561
CGTCGGT950.014.0090561
CTCCGTT2400.014.0090561
CTCCGTG9200.014.0090561
CTCCGGT5600.014.0090561
CGCCGGT1900.014.0090561
TCCCTTT2500.014.0090561
TCCCTGT8050.014.0090561
CTCCTTT3950.014.0090551
CTCCTTG9500.014.0090551
CGCCTTT758.54925E-1014.0090551
CGCCTTG2200.014.0090551
TTCCGTT1300.014.0090551
Produced by FastQC (version 0.11.5)