FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004736
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG37070.1849121280807049No Hit
GGGTGTGTTGTGTTTTTGTG24770.12355741603882006No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTG300.00591158414.0091611
CTCCTGT2150.014.0091611
TGCCGTG300.00591158414.0091611
CTCCTGG2150.014.0091611
CGCCTGT758.5310603E-1014.0091611
CGCCTGG758.5310603E-1014.0091611
TTCCGGG758.5310603E-1014.0091611
GTCCGTT758.5310603E-1014.0091611
CCTCGTT453.0166568E-514.0091611
GTCCGTG950.014.0091611
CCTCGTG601.5874139E-714.0091611
GGCCGTG453.0166568E-514.0091611
GTCCGGT453.0166568E-514.0091611
GTCCGGG758.5310603E-1014.0091611
CCGCTGT758.5310603E-1014.0091611
GCGCGGG300.00591158414.0091611
TTCCTTG1250.014.0091611
TGCCTGT1250.014.0091611
CTGCGTG453.0166568E-514.0091611
GTCCTTG1900.014.0091611
Produced by FastQC (version 0.11.5)