FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114677
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG4140.36101397839148214No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG350.001001996714.0113491
GTCCTTG300.00586071614.0113491
GCTCTTG300.00586071614.0113491
GCCCGGG300.00586071614.0113491
CTCCGGG300.00586071614.0113491
TTTTGTC558.9609057E-713.9991283
TTGTCTG558.9609057E-713.9991285
TGTTGGC558.9609057E-713.9991283
GTTTGTC558.9609057E-713.9991283
TTGTTCT852.5465852E-1113.9991284
GGTTGGC558.9609057E-713.9991283
GGGTCGG505.1680963E-613.9991285
GTGTTCG558.9609057E-713.9991284
TGCGTGT505.1680963E-613.9991287
TTTTCTT1000.013.9991285
TGTTTTC558.9609057E-713.9991283
TGTTCGT558.9609057E-713.9991285
TTTTTGC558.9609057E-713.9991283
TTTTTCT1100.013.9991284
GTTTTTC505.1680963E-613.9991283
Produced by FastQC (version 0.11.5)