FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8935561
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG199430.22318688216665972No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTG11700.014.00961
CCCCTTT3700.014.00961
CCCCTTG8550.014.00961
CTTCTTT5750.014.00961
CTCCTTT5050.014.0095991
CTCCTTG9750.014.0095991
CGCCTTT652.7777787E-814.0095991
TTCCGTT1300.014.0095991
CTCCTGT14650.014.0095991
TGCCGTG1950.014.0095991
CTCCTGG18150.014.0095991
CGCCTGT1400.014.0095991
CGCCTGG2650.014.0095991
CCTCGTT3200.014.0095991
GGCCGTT1950.014.0095991
CCGCTTT601.5889054E-714.0095991
GGCCGGT3150.014.0095991
TCGCGTG350.001013629314.0095991
TGCCTTG3800.014.0095991
TTCCTGG10100.014.0095991
Produced by FastQC (version 0.11.5)