FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013643
Sequences flagged as poor quality0
Sequence length20
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG112620.22462708254257432No Hit
GGGTGTGTTGTGTTTTTGTG57430.11454744583928295No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTT559.1125E-714.0088341
CGCCTGT1100.014.0088341
TGCCGGG1100.014.0088341
CCGCTTT559.1125E-714.0088341
CCTCGGT2200.014.0088341
CCCCGTG3050.014.0088341
GCGCTTG1100.014.0088341
GCTCTTG4400.014.0088341
CGTCTTT559.1125E-714.0088341
CTCCGTT1950.014.0088341
CGCCGGT559.1125E-714.0088341
CCGCGTG559.1125E-714.0088341
GCCCTTG4400.014.0088341
CTCCTTG5500.014.0088331
CGCCTTT401.74621E-414.0088331
TTCCGTG2100.014.0088331
TGCCGTT453.0191033E-514.0088331
CTCCTGT6850.014.0088331
CTCCTGG11700.014.0088331
TTCCGGT1450.014.0088331
Produced by FastQC (version 0.11.5)