FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468214
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG22640.15420095435678993No Hit
GGGTGTGTTGTGTTTTTGTG17630.12007786330875472No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTG652.7686838E-814.0106361
TCTCGTG652.7686838E-814.0106361
CTTCGTT652.7686838E-814.0106361
CTGCTGT652.7686838E-814.0106361
TCTCTGT652.7686838E-814.0106361
GCTCTGT1300.014.0106361
CTCCGTT652.7686838E-814.0106361
CTTCTGT1300.014.0106361
CTCCTTT905.456968E-1214.0106351
CTCCTTG1100.014.0106351
CGCCTTT350.001012353814.0106351
TTCCGTT300.005906466414.0106351
TTCCGTG401.742978E-414.0106351
CTCCTGT1450.014.0106351
TGCCGTG559.0866524E-714.0106351
CGCCTGT350.001012353814.0106351
TTCCGGT401.742978E-414.0106351
TGCCGGT350.001012353814.0106351
CCTCGTG350.001012353814.0106351
GTCCGGT350.001012353814.0106351
Produced by FastQC (version 0.11.5)