FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149838
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG4510.3009917377434296No Hit
CTGTGTGTTTTTGTGTTGTG1760.11746019033889936No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG350.001005973214.0086851
CTCCTGT401.7285961E-414.0086831
GTCCTGT300.00587924614.0086831
CCTCTGG452.9810948E-514.0086831
CTGCTGG300.00587924614.0086831
TTGTCTG852.5465852E-1113.9993335
TTTTGCT1100.013.9993334
TTTTGCG559.012565E-713.9993334
TGGTTCT559.012565E-713.9993334
GTGTCTG852.5465852E-1113.9993335
GTTTGGC852.5465852E-1113.9993333
GGTTGGC852.5465852E-1113.9993333
TTTTCTT559.012565E-713.9993335
GATGTGG559.012565E-713.9993338
TTTTCGT559.012565E-713.9993335
GTTTTCT559.012565E-713.9993334
TGGGCTT559.012565E-713.9993335
GTTGGGC852.5465852E-1113.9993333
TGGCGGG559.012565E-713.9993336
GGGCGTG852.5465852E-1113.9993336
Produced by FastQC (version 0.11.5)