FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9588470
Sequences flagged as poor quality0
Sequence length20
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG270980.2826102600310581No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTTT3550.014.0100271
CCCCTTT3550.014.0100271
CTCCGGT5450.014.0100271
CTCCTTT4700.014.0100261
CGCCTTT905.456968E-1214.0100261
CGCCTTG2400.014.0100261
CTCCTGG25600.014.0100261
TTCCGGT2000.014.0100261
TTCCGGG3450.014.0100261
GTCCGTT4000.014.0100261
GTCCGTG10850.014.0100261
GGCCGTT2000.014.0100261
CCGCTTT453.0172465E-514.0100261
CCTCGGG9600.014.0100261
TCGCGTT300.00591131914.0100261
TCGCGGT801.4915713E-1014.0100261
TGCCTTT2300.014.0100261
TTCCTGG9900.014.0100261
CCTCTTT3600.014.0100261
CCCCGTT2400.014.0100261
Produced by FastQC (version 0.11.5)